SUBA II
Search for Arabidopsis proteins where...
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Protein location inferred by to be in Add Search for proteins that have (or have not) been assigned to a specific subcellular location by direct assay. GFP and MSMS describe assignments that were made by local curators based on in-house research or literature survey. TAIR, UniProt and AmiGO describe assignments made by external curators based on direct assay evidence.
Protein location predicted by to be in Add Search for proteins that have (or have not) been assigned to a specific subcellular location by a predictor program. Assignments are based on the protein set from TAIR7 and include the following predictors: iPSORT, WoLF PSORT, Mitopred, MitoProt2, SubLoc, PeroxP, Predotar, TargetP
Protein location described in Add One aspect of SUBA involved manual literature trawling for papers related to subcellular protein localization in Arabidopsis. Use this query component to extract calls by referenced paper. If you know of a paper that does not appear to be in the list, please inform Harvey Millar (hmillar(at)cyllene.uwa.edu.au) or Joshua Heazlewood (botjlh(at)cyllene.uwa.edu.au).
Protein information keyword Add Search for/exclude proteins by keyword. A search will be conducted against the TAIR description of proteins in the SUBA database. The syntax of this search supports extended regular expressions (see this site for more information)
Numeric options: Add Filter for proteins based on various numeric data, derived from TAIR7 or calculated locally.
Underlying gene model chromosome Add Filter proteins so that only those that are translated from genes on (or not on) the specified chromosome are included
Arabidopsis Gene Identifier Add Enter a list of valid AGI's separated by new lines, spaces, commas, tabs or semi-colons. Alternative splice information may be included if desired. The search will attempt to filter results based on the list you have provided

SUBA version 2.2

The SubCellular Proteomic Database (SUBA) houses large scale proteomic and GFP localisation sets from cellular compartments of Arabidopsis. It also contains precompiled bioinformatic predictions for protein subcellular localisations. SUBA was last updated in October 2007 and is based on the TAIR7 genome annotation release. A PDF tutorial explaining how to use SUBA is available here: SUBA tutorial.

If you find this resource useful please cite one of the following publications:

Heazlewood JL, Verboom RE, Tonti-Filippini J, Small I and Millar AH. (2007) SUBA: the Arabidopsis Subcellular Database. Nucleic Acids Res. 35:D213-D218. (PubMed)

Heazlewood JL, Tonti-Filippini J, Verboom RE, Millar AH. (2005) Combining experimental and predicted datasets for determination of the subcellular location of proteins in Arabidopsis. Plant Physiol. 139(2):598-609. (PubMed)

version 2.0.0